Possible Interaction: Zinc Sulfate and Beta-Cryptoxanthin

“Culture with CRP (10(-7) M) plus zinc ((10(-5) M) for 24 or 72 h caused a significant increase in caspase-3 mRNA expression in the presence or absence of M-CSF and RANKL as compared with the value for each chemical alone.”

“Nuclear factor of activated T cells c1 (NFATc1) mRNA expression was significantly decreased after culture with CRP plus zinc in the presence or absence of M-CSF and RANKL for 72 h as compared with each chemical alone, while NF-kappaB mRNA expression was not significantly changed.”

“The mRNA expression of tartrate-resistant acid phosphatase (TRACP) and cathepsin K was significantly decreased after culture with CRP plus zinc in the presence or absence of M-CSF and RANKL for 72 h as compared with CRP or zinc alone.”

“The number of osteoclastic cells was significantly decreased after culture with the combination of CRP (10(-7) M) and zinc (10(-5) M) in the presence or absence of M-CSF and RANKL for 24 or 72 h as compared with the value for CRP or zinc alone.”

“This study demonstrated that the combination of CRP and zinc has potent suppressive effects on osteoclastic cells in vitro.”

“We investigated whether the effect of beta-cryptoxanthin CRP ) on osteoclastic cells formed in the mouse marrow culture system in vitro is enhanced by culture with zinc.”

“Also, … of beta-cryptoxanthin (10(-9) …) plus zinc (10(-6) M) caused a remarkable increase in alkaline phosphatase activity and deoxyribonucleic acid (DNA) in the femoral-diaphyseal and -metaphyseal …, while their application alone did not have an effect on the enzyme activity or DNA content in the femoral tissues.”

“The … beta-cryptoxanthin (… zinc (10(-6) M) in increasing calcium content, alkaline phosphatase activity, and DNA content in the femoral-diaphyseal and -metaphyseal tissues was completely prevented … (10(-6) M), an inhibitor of protein synthesis, or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DBR), an inhibitor of transcriptional activity.”

“This study demonstrates that the combination of beta-cryptoxanthin and zinc at a lower concentration has a synergistic effect on bone components in vitro.”