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“An in vitro experiment showed that the binding affinity to warfarin became stronger when HSA was glycated, while HSA binding to heparin was not significantly influenced by glycation.”
Journal of pharmaceutical sciences • 2009 | View Paper
“The ability of HSA to form ternery complexes with other neutral molecules such as warfarin , which also binds within the subdomain IIA pocket, increases our understanding of the binding dynamics of exogenous drugs to HSA.”
“The two highest levels of oxidation of HSA (CT10-HSA, CT50-HSA) resulted in conformational changes with an increased exposure of hydrophobic regions, decreased high-affinity bindings of warfarin and ketoprofen and a reduced esterase-like activity.”
“The binding of either dicoumarol or warfarin to HSA quenches the native tryptophan fluorescence of the protein.”
“The binding of warfarin to HSA is also accompanied by a 7-fold increase in the fluorescent yield of the drug and a shift of its fluorescence emission maximum from 400 mµ to 390 mµ. This suggests that warfarin is bound to a hydrophobic area of the HSA molecule.”
“The retention of warfarin on the entrapped HSA was at least 1.7-fold higher than has been obtained under comparable support and mobile phase conditions when using covalent immobilization.”
“Furthermore, the binding ability of HSA to warfarin and ketoprofen was more effectively decreased by methylglyoxal modification than the other aldehydes.”