Possible Interaction: Vitamin E and Arachidonate

“ Tocopherol regeneration declined with increasing oxidation time induced by arachidonate , and after 30-60 min virtually no regeneration could be observed, suggesting that the precursor molecule was unstable.”

“The present study suggests that concentrations of vitamin E as low as 50-100 microM inhibit cyclooxygenase-independent platelet aggregation when combined with an inhibitor of the arachidonate pathway.”

“For example, incubation of human platelets with diamide or arachidonate resulted in oxidation of alpha-tocopherol and the alpha-tocopherolquinone produced accounted for 11.8 and 30.6%, respectively of the alpha-tocopherol lost.”

“ alpha-tocopherol potentiated the incorporation of labelled arachidonate in the platelet phospholipids.”

“All-trans retinoate, d-α-tocopheryl acid succinate , and dihomogeranoate are not activated themselves, but at low concentrations extensively inhibit activation ofcis-acids (oleate,cis-vaccenate, petroselenate, linoleate, linolenate, arachidonate ) and thetrans-diunsaturated linelaidate but do not affect activation of palmitate ortrans-monounsaturated acids elaidate andtrans-vaccenate.”

“Collectively, these results suggest that vitamin E potentiates arachidonate release through the direct and/or indirect modulation of cPLA2 activity.”

“Exposure of myocyte cultures to free radical generating solutions resulted in increased levels of conjugated dienes … increased release of [3H]arachidonate and lactate dehydrogenase compared to control values after 1 h. alpha-Tocopherol treatment attenuated the increase in conjugated … levels, and the release of [3H]arachidonate and lactate dehydrogenase.”

“Pretreatment with either MPSS or a‐tocopherol and Se reduced the trauma‐induced release of total FFA including arachidonate in the injured spinal cord tissue.”