Possible Interaction: Urea and Polypeptides

“The production of biologically active eIF-4 alpha only occurs when the polypeptide is totally reduced during solubilization of the inclusion bodies in 8 M urea.”

“The presence of urea during solubilization of collagenous samples for SDS PAGE had a marked effect upon mobility … certain rabbit antisera, antibodies from several human sera and binding of concanavalin A. When samples were solubilized with urea by … collagenous polypeptides was retarded relative to samples … to urea without heating.”

“The specific activity of individual polypeptides extracted by urea fell an average of 25% during the 10-day chase.”

“This difference in magnitude is caused by the temperature-dependent negative heat effect of additional binding of urea to the polypeptide chain of the protein upon its unfolding, which decreases the positive enthalpy of heat denaturation and increases the negative enthalpy of cold denaturation.”

“The polypeptide resists denaturation by guanidinium chloride or urea but is totally inactivated by treatment with reducing agents.”

“Once associated with the lipid bilayer, the vimentin polypeptide resists urea treatment, suggesting that it has become an integral constituent associated with part of the membrane.”

“Sodium dodecylsulfate or urea dissociated cross-linked informofers into oligomeric polypeptides.”

“ Urea solubilized preferentially a polypeptide with a mol.”

“This observation strongly suggests that urea interacts with sites common to the four different proteins, such as components of the polypeptide backbone.”

“The hypocalcemic and leukocytosis-promoting activities of P-I were eliminated by treatments with heat, HCl, NaOH, urea and nitrous acid and weakened with nitrogen mustard.”