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“For the copper metallochaperone Atx1 from Synechocystis PCC 6803 (a cyanobacterium), Saccharomyces cerevisiae and humans unfolding in urea results in a decrease in the Cu(I) affinity from (4-5) × 10(17) M(-1) to (1-3) × 10(16) M(-1) at pH 7.”