Possible Interaction: Trypsin and Tryptophan

“Both the Trp at the P(2)' position and deletion at the P(20)' position are thus likely required for the inhibition of factor Xa, trypsin , and factor IXa, while these two positions do not affect factor X activation initiated by the factor VIIa-tissue factor complex.”

“Superoxide dismutase, a scavenger of superoxide, and free-radical scavengers benzoate and tryptophan inhibited the glomerular chemiluminescence response to trypsin and chymotrypsin.”

“However, it was found from cyanogen bromide treatment that there is a marked difference in the location of one of the methionine residues, while the tryptophan-containing peptides liberated after trypsin digestion have different mobilities on peptide maps.”

“However, the rate constant for quinonoid intermediate formation from L-tryptophan is reduced by at least 400-fold by treatment with trypsin.”

“In the presence of L-tryptophan , cleavage by trypsin between residues 54-55 and 84-85 was retarded, even when a previous hydrolytic event elsewhere in the protein had occurred.”

“Thus, there is an extensive conformation-dependent energy transfer between tryptophans in trypsin.”

“This analysis indicates that hen lysozyme … an essential Trp residue, that bovine trypsin … Trp residue adjacent to the key catalytic Ser and other pathways initiated by excitation of Tyr and Cys, that … RNase A is not an important inactivating reaction, and that aromatic residues in subtilisn Carlsberg are photosensitive.”