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“During the log phase of growth, MSC proliferated with a two fold population at 30 h. MSC can be ex vivo expanded by successive cycles of trypsinization , seeding, and culture.”
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae • 2002 | View Paper
“We report significantly reduced MSC EV vascularization bioactivity, as measured by an endothelial cell gap closure assay, with increasing passage in culture by trypsinization , especially beyond passage 4.”