Possible Interaction: Trehalose and Urea

“Our parallel experimental results explicitly elucidate that trehalose strongly offset the deleterious actions of urea on CT at 1:2 molar ratio of trehalose and urea, however, trehalose (1 M) some how failed to counteract the perturbation effects of urea (3-6 M) on CT.”

“… trehalose … urea on α-chymotrypsin (CT) through the calorimetric data (transition temperature (T(m)), enthalpy change (ΔH), heat capacity change (ΔC(p)) and Gibbs free energy …:2 ratio of trehalose and urea, as well as various urea concentration (up to 6 M) in the presence of 1 M trehalose.”

“In the present work, we show that four different sugars, namely fructose, glucose, sucrose, and trehalose , counteract the effect of urea , tetramethylurea, sodium perchlorate, guanidinium chloride, and guanidinium thiocyanate despite the chemical and structural differences of those destabilizing agents.”

“Implications of these results for the molecular explanations of the counteracting mechanism of trehalose on urea induced protein denaturation are discussed.”

“To provide the underlying mechanism of the inhibiting effect of trehalose on the urea denatured protein, we perform classical molecular dynamics simulations of N-methylacetamide (NMA) in aqueous urea and/or trehalose solution.”

“Considerable experimental evidence indicates that trehalose can counteract the denaturing effects of urea on proteins.”

“ Trehalose was also efficient to prevent an irreversible inactivation induced by urea.”