Possible Interaction: Threonine and Trypsin

“The percent …, activities of trypsin , lipase and amylase, weights and protein contents of hepatopancreas and intestine, folds heights, activities of alkaline phosphatase (AKP), γ- glutamyl transpeptidase and …-pyruvate transaminase (GPT) activities in hepatopancreas, and 4E-BP2 gene expression in muscle, hepatopancreas and intestinal segments were significantly enhanced by Thr (…).”

“The electrophoretically pure preparation which inhibits trypsin in a specific manner is a relatively small peptide containing 5 Asp, 4 Thr , 1 Ser, 11 Glu, 6 Pro, 6 Gly, 5 Ala, 2 Val, 10 (Cys)1/2, 3 Ile, 2 Phe, 7 Lys, 3 Arg and 1 Try.”

“Substitution of residue 42 with alanine and residue 58 with alanine or valine in the presence of threonine 195 results in trypsin variants that are 102–104‐fold less active than wild type in kcat/KM but >106‐fold more active than the Ser 195 → Thr single variant.”