Possible Interaction: Threonine and Sirolimus

“Both wortmannin and rapamycin inhibited isoflurane-induced phospho-4E-BP1 (Ser 65) and phospho-P70s6k ( Thr 389) and HIF-1α expression.”

“…,RAPA (especially at 1.2 mg/kg BW) …, β-casein, and amino acid contents (Asp, Thr , Ser, Glu, Gly, Ala, Cys, Val, Met, Ile, Leu, Tyr, Lys, His, Arg, and Pro) of crop … of albumin, total protein, and uric acid in the serum of breeding pigeons (P < 0.05).”

“Furthermore, l-threonine induced phosphorylation of raptor and rictor binding to mTOR was completely inhibited by 24 h treatment with rapamycin (mTOR inhibitor); however, a 10 min treatment with rapamycin only partially inhibited rictor phosphorylation.”

“In addition, rapamycin blocked l-threonine-induced increases in mRNA expressions of trophoectoderm and mesoderm marker genes and mESC proliferation.”

“ l-Threonine induced translocation of rictor from the membrane to the cytosol/nuclear, which blocked by pretreatment with rapamycin.”

“ Rapamycin prevented the phosphorylation of p70S6K at the Thr 389 site (which correlates with enzyme activity), reduced ERK1/2 phosphorylation, but had no effect on the Thr 421/Ser 424 site or on p38(MAPK) phosphorylation.”

“Inhibition of threonine incorporation into cell wall and leucine incorporation into cellular protein was observed at relatively higher concentrations of rapamycin.”

“…,RAPA (especially at 1.2 mg/kg BW) …, β-casein, and amino acid contents (Asp, Thr , Ser, Glu, Gly, Ala, Cys, Val, Met, Ile, Leu, Tyr, Lys, His, Arg, and Pro) of crop … of albumin, total protein, and uric acid in the serum of breeding pigeons (P < 0.05).”

“Inhibition of protein synthesis by rapamycin or cycloheximide resulted in the phosphorylation of BiP on threonine residues while ER stress induced by tunicamycin or heat shock caused the fast dephosphorylation of the protein.”