Possible Interaction: Threonine and Aspartate

“It seemed that threonine blocked this synthesis by inhibiting the conversion of aspartate.”

“Additions of aspartate with asparagine, and threonine with vitamin B1 (as thiamine hydrochloride) resulted in significant increases in final antibody concentrations.”

“Aspartate or threonine can not occupy the kinase active site simultaneously; this conclusion is consistent with the direct competitive inhibition of aspartate by threonine observed in steady-state kinetic studies.”

“The formation of the quaternary aspartate-containing adduct was unexpected, since the presence of threonine was expected to prevent access of the aspartate to the active site; most significantly however, the the sum of the numbers of aspartate plus threonine molecules incorporated per active site is one.”

“We believe that this shows direct steric overlap between the metal-adjacent binding sites for aspartate and threonine.”