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“Typical ribosomal inhibitors such as tetracycline , chloramphenicol, phenylboric acid, fusidic acid have been shown to inhibit the template-free synthesis of polypeptides.”
“The 34,000 dalton polypeptide along with another plasmid-encoded polypeptide of 18,000 daltons function in an ATP-independent manner to prevent the accumulation of tetracycline by the cell.”
“Five discrete polypeptide species were synthesized by minicells carrying plasmid 219; exposure of minicells or parent bacteria to Tc resulted in specific and reproducible changes in polypeptide synthesis patterns.”