Possible Interaction: Tempol and 3-Nitrotyrosine

“For instance, 10 μM tempol was able to inhibit by 90% the yield of 290 μM 3-nitrotyrosine produced from 370 μM RNase.”

“ Tempol significantly (P ≤ 0.01) reduced ATP expression, 3-NT , and ROS/RNS expression in the urinary tract of CYP-treated rats.”

“Furthermore, tempol reduced the levels of serum thiobarbituric acid reactive substance (TBARS) and 3-nitrotyrosine content in mesenteric arteries.”

“With the tempol pre-treatment, the immunoreactivity of COX-2 and nitrotyrosine in paraffin-embedded tissue slices was profoundly decreased.”

“ Tempol treatment reduced nitrotyrosine staining in both the iron and non-iron groups.”

“Consistent with that, the protection of mitochondrial respiratory function by tempol is associated with a decrease in 3-NT levels in mitochondrial proteins also similar to the previously reported antioxidant actions of tempol in traumatically-injured spinal cord mitochondria.”

“ Tempol significantly antagonized the respiratory effects of SIN-1 in parallel with an attenuation of 3-NT levels.”

“Administration of a single 300 mg/kg intraperitoneal dose of tempol 15 mins after TBI produced a complete suppression of PN-mediated oxidative damage ( 3-nitrotyrosine , 3NT) in injured cortical tissue at 1 h after injury.”

“Accordingly, tempol decreased the yields of BSA-cysteinyl and BSA-tyrosyl/tryptophanyl radicals, as well their decay products such as protein-3-nitrotyrosine.”

“In parallel, tempol decreased the levels of protein-3-nitrotyrosine in the injured tissues, suggesting that it interacted with nitric oxide-derived oxidants such as nitrogen dioxide and peroxynitrite.”

“The degree of nitrotyrosine and PARS staining was markedly reduced in tissue sections obtained from CII-treated rats that had received tempol.”

“ Tempol also reduced the appearance of nitrotyrosine and poly (ADP-ribose) synthetase immunoreactivity in the colon as well as the up-regulation of ICAM-1 and P-selectin.”