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“The principle of the assay is the inhibition of trypsin by tannins and the subsequent reversal of that inhibition when other tannin-binding proteins are added (indirect assay).”
Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology • 1999 | View Paper
“ Tannins inactivated trypsin the most, alpha-amylase to a lesser extent and lipase the least and as a consequence lowered the digestion of amino acids the most, starch to a lesser extent and lipid the least.”
The British journal of nutrition • 1991 | View Paper
“The inhibitory effect of tannins on trypsin (EC 3.4.21.4), alpha-amylase (EC 3.2.1.1) and lipase (EC 3.1.1.3) activities in vitro also increased with the increase in degree of polymerization.”
The British journal of nutrition • 1988 | View Paper
“Significant increases were obtained in trypsin and amylase activities as tannin levels increased, the lipase activity of T0 and T1 was significantly higher than T2 and T3.”