Possible Interaction: Sphingosine 1-Phosphate and Colforsin

“In Baby Hamster Kidney (BHK) cells expressing wild-type human CFTR, S1P (1μmol/L) attenuates forskolin-stimulated , CFTR-dependent iodide efflux.”

“Pre-exposure to S1P markedly inhibited the augmentation of cAMP accumulation induced by forskolin.”

“ SPP markedly inhibited forskolin-stimulated cAMP accumulation in a dose-dependent manner and caused a remarkable elevation of intracellular calcium, both effects being sensitive to pertussis toxin treatment.”

“Binding of SPP to Edg-1 resulted in inhibition of forskolin-stimulated cAMP accumulation, in a pertussis toxin–sensitive manner.”

“Furthermore, SPP inhibited forskolin-stimulated cAMP accumulation in HEK cells and increased binding of guanosine 5′-3-O-(thio)triphosphate to HEK cell membranes.”

“In the presence of forskolin , however, S1P significantly inhibited cAMP accumulation at a lower concentration (1–100 nm) of S1P in a manner sensitive to PTX in the Edg-1-transfected cells but not in either the Edg-3 or AGR16-transfected cells.”

“In a CHO clone stably expressing EDG1 receptor (CHO-EDG1 cells), SP induced increases in the production of inositol phosphates and the [Ca2+] i and inhibited forskolin-induced increase in the cellular cAMP content, all in a manner sensitive to pertussis toxin.”

“In Chinese hamster ovary cells heterologously expressing EDG-8, S1P inhibited forskolin-induced cAMP accumulation and activated c-Jun NH2-terminal kinase.”