Possible Interaction: Sphingosine 1-Phosphate and Calcium Supplement

“In both primary rat aortic vascular smooth muscle cells and HEK293 cells, the S1P content in HDL particles increased intracellular calcium concentration, which was mediated by S1PR1.”

“Both LPA (20 microM) and S1P (10 microM) also stimulated increases in intracellular calcium concentration in HTM cells.”

“ SPP markedly inhibited forskolin-stimulated cAMP accumulation in a dose-dependent manner and caused a remarkable elevation of intracellular calcium , both effects being sensitive to pertussis toxin treatment.”

“Here, we present evidence that SPP activates a G protein-coupled receptor in the plasma membrane of various cells, leading to increase in cytoplasmic Ca concentration ([Ca]), inhibition of adenylyl cyclase, and opening of G protein-regulated potassium channels.”

“Thus, in addition to … effect on mobilization of calcium, SPP can increase the level of PA, most likely via activation of phospholipase D. We suggest that SPP mediates the effect … sphingosine on PA accumulation in Swiss 3T3 fibroblasts and may regulate cellular proliferation by affecting multiple transmembrane signaling pathways.”

“Similar to haCER1 overexpression, Ca2+(o ) increased the levels of SPH and S1P , and this was attenuated by haCER1 knockdown.”

“ Sphingosine 1-phosphate ( S1P) evoked an increase in [Ca2+]i in both a calcium containing and a calcium-free buffer.”

“ S1P inhibited the platelet aggregation induced by the TRAP peptide (6.75 w M), noradrenaline (NA; 12.5 w M) and the Ca 2 + ionophore (5.0-9.5 w M).”

“Divalent ions such as Ca2+ and Mg2+ promote the adherence of Candida spp.”

“Sphingosine-1-phosphate SPP ) induces a variety of cellular responses, including Ca2+ signaling, proliferation, and inhibition of motility, apparently by acting at specific G protein coupled receptors.”