Possible Interaction: Serine and Aspartate

“All mutants exhibited similar plasma membrane expression when compared with WT SLC1A2, but substitutions of serine by aspartate or asparagine completely abolished substrate transport.”

“Substitution of glycine, glutamine or serine attenuated the extent of block whereas the negatively charged aspartate enhanced it, consistent with the side chain of the native asparagine at this position contributing to a blocking site for intracellular Mg2+.”

“Calculations suggested that substitution of aspartate by serine would result in decreases of the pK(a) values of most of the histidine residues in the protein.”

“Turnover rates of the putative neurotransmitters aspartate , glutamate, and GABA) were 2–20‐fold higher than those of alanine and serine , and generally consistent with the proposed neurotransmitter functions for these amino acids.”

“Substitution of aspartate for even one of these serines abolishes rapamycin responsiveness and increases rapamycin resistance without affecting either of the other two Gln3 localization responses.”

“ L-serine addition resulted in a substantial transient increase in L-aspartate , which is, rather than L-glutamate, the major metabolite for short-term storage of ammonia derived from degradation of L-serine.”

“Exchange of a highly conserved serine next to the nuclear localization signal by aspartate , which may mimic phosphorylation, resulted in a decreased nuclear import (SPL8), while DNA binding in vitro was abolished completely.”

“ Aspartate substitution for serine 160 inhibits nuclear localization throughout the cycle.”

“The reductions in the response threshold for aspartate by the replacements with serine , isoleucine, and proline were less than 1, about 2, and more than 5 orders of magnitude, respectively.”