Possible Interaction: Quinacrine and Arachidonate

“The lysosomotropic agents; chloroquine, quinacrine , ammonium chloride and methylamine all prevented the depression of hydrogen peroxide production and arachidonate release but did not alter the depression of phagocytic function.”

“Both chloroquine and quinacrine , and to a lesser extent hydroxychloroquine, inhibited arachidonate release and eicosanoid formation induced by phorbol diester.”

“The release of arachidonate was blocked by quinacrine which is a specific inhibitor of phospholipase A2.”

“The inhibitors of phospholipase A2, quinacrine and p-bromophenacyl bromide, decreased the release of [3H]arachidonate in a dose-related manner.”

“Inhibition of phospholipase A2 with para-bromophenacyl bromide, dexamethasone or quinacrine , alone or in combination, blocked arachidonate release by 50%-60% but had no effect on EDRF production as assessed by cyclic GMP accumulation in mixed- or co-cultures of endothelial and vascular smooth muscle cells.”

“Hence, although mepacrine reduces prostaglandin production by decreasing the lipolytic release of arachidonate from medullary lipids, its inhibitory effect on prostaglandin cyclooxygenase activity is substantial and appears to contribute significantly to its overall inhibition of prostaglandin generation in kidney medulla.”

“Inhibition of PGE and cAMP accumulation by tetracaine and mepacrine was also overcome by arachidonate.”