Possible Interaction: Quercetin and Temozolomide

“Conclusion:Combined treatment with TMZ and quercetin efficiently suppressed human glioblastoma cell survival in vitro.”

“TMZ or quercetin anole did not affect caspase-3 activity and cell apoptosis, while TMZ combined with quercetin significantly increased caspase-3 activity and induced cell apoptosis.”

“When combined with quercetin (30 μmol/L), TMZ (100 μmol/L) significantly inhibited the cell viability, and the inhibition of TMZ (200 and 400 μmol/L) was enhanced.”

“Co-administration of Temodal (100 μM) with a low quercetin concentration (5 μM) resulted in a very significant induction of autophagy; however, after treatment with quercetin at a higher concentration (30 μM), apoptosis became the primary mechanism of cell death.”

“During combined administration of both drugs, Temodal attenuated the cytotoxic effects of quercetin.”

“Our results indicate that Temodal and quercetin are synergistic inducers of programmed cell death, better together than applied separately.”

“The highest number of dead cells was observed after simultaneous administration of both drugs or after pre-incubation with Temodal followed by treatment with quercetin.”

“At a low (5muM) drug concentration, quercetin potentiated a pro-autophagic effect of Temozolomide , while after treatment with a higher drug concentration (30muM), autophagy switched to apoptosis.”

“Our results indicate that quercetin acts in synergy with Temozolomide and when used in combination rather than in separate pharmacological application, both drugs are more effective in programmed cell death induction.”

“ Temozolomide administered with quercetin seems to be a potent and promising combination which might be useful in glioma therapy.”

“ Temozolomide attenuated the toxic effect of quercetin.”

“ConclusionThis study demonstrates that Qct can sensitize cells to TMZ and that the mechanisms of sensitization involve modulation of p53 family members.”

“Here … that Qct also … to TMZ and propose a mechanism that involves the modulation of a truncated p53 family member, ΔNp73.MethodsDB-1 melanoma (p53 wildtype), and SK … (p53 mutant) cell lines were treated with TMZ (400 μM) for 48 hrs followed by Qct (75 μM) for 24 hrs.”