Possible Interaction: Proline and Thioproline

“The nitrosation of thioproline was estimated to be 1000-fold that of proline in the human body.”

“Separation of the influence of metabolism from … the use of proline analogues showed that thioproline … proline uptake by 80%, primarily by reducing the conversion of proline to glutamic acid, whereas 3,4-dehydroproline … to glutamic acid but principally appeared to alter the affinity of the transport system for proline.”

“The active part of the proline molecule is the α-imino group; substitutions in this group, such as 1-thiazolidine-4-carboxylic acid and glycylproline, annul the activity of proline.”

“Both analogs gave higher proline accumulation after 6 and 10 days (68 and 179% after 6 days with A2C at 25 and 50 μM, respectively, and 111% with T4C added at 100 μM).”

“ T4C (1 millimolar) inhibited proline (10 millimolar) -dependent 0(2) uptake an average of 67%.”

“ Proline antagonizes the incorporation of " thioproline " into protein.”

“ Proline antagonizes the incorporation of “ thioproline ” into protein.”

“The effectiveness of thioproline as a proline analogue is attributed to its ability to interfere with the utilization of proline for protein synthesis and to mimic proline in its function of being incorporated into proteins.”

“Hence, these experiments show that TZCA may be used as a competitive inhibitor to the incorporation of proline into ribosomes in a cell-free system.”