Possible Interaction: Proline and Aspartate

“Infusion of the Glu transport inhibitor l‐trans‐pyrrolidine‐2,4‐dicarboxylic acid (200 µM) increased resting Glu and Asp levels by 300–500% without altering electrically stimulated changes in Glu and Asp concentration.”

“7 The putative glutamate uptake current was further characterized; it was blocked by the competitive uptake blockers D‐aspartate (0.5 mM) and L‐trans‐pyrrolidine‐2,4‐dicarboxylic acid (PDC; 0.5 mM), and by replacement of sodium with lithium.”

“ PDC 10 mM perfusion produced an increase in the extracellular output of glutamate and aspartate , and a decrease in that of ascorbate.”

“In line with its enhancement of paired-pulse facilitation, 30 microM proline reduced both the K+-evoked release of glutamate and aspartate from CA1 slices and the release of glutamate and aspartate from CA1 synaptosomes evoked by 4-aminopyridine.”

“Indeed, the effects of proline on glycogen synthesis, lipogenesis and glutamate and aspartate content were faster.”

“The reductions in the response threshold for aspartate by the replacements with serine, isoleucine, and proline were less than 1, about 2, and more than 5 orders of magnitude, respectively.”

“In this concentration range heroin increases the rate of accumulation in the free amino acid "pool" of aspartate , glutamate, and alanine, inhibits the accumulation of lysine and proline , and has no effect on the accumulation of five other amino acids tested.”