Possible Interaction: Probenecid and Riboflavin

“ Benemid can inhibit transport of both PAH and riboflavin.”

“The membrane transport inhibitor amiloride, but not 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, 4-acetamide-4'-isothiocyanostilbene-2,2'-disulfonic acid, furosemide, or probenecid , inhibited RF uptake in a competitive (inhibitory constant = 0.48 mM) and reversible manner.”

“ Probenecid decreased the renal clearance of riboflavin , and this effect was directly related to the serum concentration of the inhibitor.”

“It appears that probenecid also inhibits the specialized transport process responsible for the intestinal absorption of riboflavin in man.”

“Oral administration of probenecid prior to intramuscular injection of FMN increased the apparent “half-life” of the initial rapid phase of riboflavin excretion.”

“The urinary recovery of riboflavin following oral or intramuscular administration of the vitamin was usually somewhat decreased by probenecid.”

“The uptake of RF by BBM vesicles was, however, inhibited by probenecid and organic anion transport inhibitors, 4,4-diiso-thiocyanatostilbene-2,2-disulfonic acid (DIDS, 1 mM) and 4-acetamido-4-isothiocyanatostilbene-2,2-disulfonic acid (SITS, 1 mM).”

“While the transmembrane transport inhibitors 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS), 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and furosemide had no effect on RF uptake, amiloride and probenecid suppressed RF uptake in a dose-dependent fashion.”