Possible Interaction: Potassium Chloride and Genistein

“In addition, genistein , resveratrol and 17beta-estradiol also could reduce the contractile responses of ACh and KCl , and shift their cumulative concentration-response curves rightward.”

“Both U46619 (30 nM) and KCl (50 mM) increased MLC20 phosphorylation levels, which were inhibited by genistein and daidzein.”

“Furthermore, both genistein and daidzein decreased the amount of GTP RhoA activated by either U46619 or KCl.”

“The cumulative additions of U46619 or KCl increased vascular tension in a concentration-dependent manner, which were inhibited by pretreatment with genistein or daidzein.”

“ Genistein concentration-dependently relaxed histamine (30 microM)-, carbachol (0.2 microM)-, KCl (30 mM)- and leukotriene D4 (10 nM)-induced precontractions and inhibited cumulative histamine- and carbachol-induced contractions in a non-competitive manner.”

“ Gen (50 microM) also inhibited by 60% the [Ca(2+)](i) response to 50 mM KCl , indicating a direct interaction with voltage-sensitive, L-type Ca(2+) entry channels.”

“In addition, genistein could reduce the contractile responses of NA, KCl and CaCl2, and shift their cumulative concentration-response curves rightward. (”

“ Genistein further reduced Ca(2+) influx in response to KCl in the presence of nitrendipine, omega-conotoxin GVIA and omega-agatoxin IVA, simultaneously.”

“Our results suggest that genistein inhibits glutamate release and Ca(2+) influx in response to KCl independently of tyrosine kinase inhibition, and that tyrosine kinases and phosphatases are not key regulators of glutamate release in hippocampal nerve terminals.”

“We found that lavendustin A and herbimycin A did … glutamate release stimulated by 15 mM KCl, but genistein , also a broad range inhibitor of tyrosine kinases … inhibit the intracellular Ca(2+) concentration response to KCl and, concomitantly, decreased glutamate release evoked by … same stimulus, in a dose-dependent manner.”

“At 100 microM genistein also inhibited the contractile effects of PdBu and KCl.”

“ Genistein , but not tyrphostin A23 or diadzein (30 microM), attenuated significantly the contraction induced by KCl (p < 0.05).”

“ Genistein (20 microM), kaempferol (10 microM), quercetin (15 microM), verapamil (70 nM), nifedipine (10 nM) and forskolin (0.5 microM) inhibited the concentration-response curve to CaCl2 in medium supplemented with 33, 60 or 90 mM of KCl.”

“ Genistein (IC50: 20.2 +/- 1.0 microM, n = 11), kaempferol (IC50: 10.1 +/- 0.8 microM, n = 8) and quercetin (IC50: 13.2 +/- 0.5 microM, n = 8) relaxed the tonic contraction induced by KCl (60 mM) in a concentration-dependent way.”

“However, the inhibitory effect of genistein on CaCl2-induced contraction was inversely related to the concentration of KCl in the medium.”