Possible Interaction: Phospholipids and Trypsin

“ Trypsin treatment of the cultured macrophages completely inhibited apoA-I-mediated efflux of cholesterol and phospholipid.”

“ Trypsin treatment of granules enhanced the rate of fusion of phospholipid vesicles with granules, suggesting that access to phospholipids in the granule membrane may be modulated by granule proteins or that a fusogenic protein factor in the granule membrane is activated by trypsin treatment.”

“Accordingly, pretreatment of isolated hepatocytes with trypsin prevented the methylation of phospholipids from exogenous AdoMet by 60-80%, whereas it was almost without effect when exogenous methionine was the methyl donor.”

“Proteins and phospholipids from mitochondrial membranes proved to be more susceptible to the effect of trypsin and phospholipase D. Kinetics of cytochrome C desorption from membranes and mitochondria demonstrated that capacity of these membranes to confine the exogenous proteins was altered in hepatitis.”

“Polyacrylamide-gel electrophoresis indicated that removal of phospholipid allowed much more extensive degradation of constituent polypeptides by trypsin.”

“The exchange of methylated phospholipids was reduced after pretreating both microsomes and mitochondria with trypsin , indicating the involvement of membrane proteins from both organelles.”

“However, removal of the delta and epsilon subunits by treatment of the enzyme with trypsin prevented binding to phospholipid.”