Possible Interaction: Phospholipids and N-Acetylaspartate

“ DnaA interacts with acidic phospholipids , such as cardiolipin, and its activity is regulated by these phospholipids.”

“In vitro, acidic phospholipids reactivate DnaA , and in vivo depletion of acidic phospholipids, results in growth arrest.”

“ Acidic phospholipids have been shown to promote dissociation of bound nucleotides from Mycobacterium tuberculosis DnaA (DnaA(TB)) purified under denaturing conditions [Yamamoto et al., (2002) Modulation of Mycobacterium tuberculosis DnaA protein-adenine-nucleotide interactions by acidic phospholipids.”

“ Acidic phospholipids … DnaA binding to ATP, and here we examine the effects of various phospholipids on DnaA binding to oriC. Among the phospholipids in E. coli … phosphatidylglycerol containing unsaturated fatty acids inhibited binding more potently than did synthetic phosphatidylglycerol containing saturated fatty acids, suggesting that membrane fluidity is important.”

“Thus, acidic phospholipids seem to inhibit DnaA binding to both oriC and adenine nucleotides in the same manner.”

“Accumulating evidence, both in vitro and in vivo, that supports the importance of membrane phospholipids influencing the initiation activity of DnaA is reviewed.”