Possible Interaction: Phosphatidic Acid and Sphingosine 1-Phosphate

“Most importantly, SPP stimulated phosphatidic acid formation, which was maximal after 2 min and decreased within 8-10 min.”

“The decrease of phosphatidic acid level after 8-10 min incubation with SPP was accompanied by a parallel increase of diacylglycerol production, which was abolished in the presence of butan-1-ol.”

“The concentration dependence for the formation of PA induced by SPP correlated with its effect on DNA synthesis.”

“Thus, in addition to its … on mobilization of calcium, SPP can increase … level of PA , most likely via activation of phospholipase D. We suggest that SPP mediates the effect of sphingosine on PA accumulation in Swiss 3T3 fibroblasts and may regulate cellular proliferation by affecting multiple transmembrane signaling pathways.”

“Conversely, S1P , but not SPC, increased the level of phosphatidic acid.”

“ Sphingosine-1-phosphate triggers dual signal transduction pathways of activation of phospholipase D leading to increases in the levels of phosphatidic acid and mobilization of calcium from internal stores.”

“ Sphingosine-1-phosphate itself induces a more rapid increase in phosphatidic acid , thus suggesting that it may mediate the effects of sphingosine on phosphatidic acid accumulation.”

“The concentration dependence for the formation of phosphatidic acid induced by sphingosine-1-phosphate correlates with its effect on DNA synthesis.”

“Thus, in addition to … mobilization of calcium, sphingosine-1-phosphate can … of phosphatidic acid , most likely via activation of phospholipase D. We suggest that sphingosine-1-phosphate mediates the effect of sphingosine … acid accumulation in Swiss 3T3 fibroblasts and may regulate cellular proliferation by affecting multiple transmembrane signaling pathways.”

“Addition of lysophosphatidylcholine increased the labelling of phosphatidylcholine, whereas addition of glycerol 1-phosphate increased the labelling of phosphatidic acid and diacylglycerol.”