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“Decapitation with prior administration of CO2 or pentobarbital sodium increased plasma ACTH levels approximately 13- and 2-fold, respectively, when compared with decapitation without anesthesia.”
American journal of physiology. Endocrinology and metabolism • 2005 | View Paper
“The following studies were performed to see if pentobarbital would prevent ether activation of ACTH release, measured indirectly by plasma corticosterone levels.”