Possible Interaction: Ovalbumin and Methacholine

“ OVA challenge resulted in an increase in AHR to methacholine , as well as increased airway eosinophilia and TH2 cytokine production.”

“Both HiTr and OVA intervention significantly increased airway resistance to aerosolized methacholine measured by whole body plethysmography.”

“RESULTS: The tracheal response of the group S guinea pigs to both methacholine and ovalbumin was significantly higher than the response of the controls (p<0.01 for both cases).”

“Nevertheless, after OVA provocation, airway responsiveness to methacholine was higher in males compared with females and OVX mice.”

“ Ovalbumin sensitization induced: (a) an inflammatory reaction with eosinophil and neutrophil influx in BALF and lung; and (b) an increased bronchial responsiveness to MCh.”

“Airway responsiveness to methacholine , measured 24-h post multiple intranasal OVA challenges, was significantly increased compared to time matched PBS-controls (P<0.05).”

“The … of methacholine causing a 100% increase of airway resistance (PD 100 airway resistance) was 0.54 +/- 0.19 mg/mL and 1.17 +/- … mg/mL using OVA and ozone exposure alone, which had decreased to 0.49 +/- 0.18 mg/mL when both OVA and ozone … given simultaneously.”

“In control animals, OVA evoked airway hyperreactivity to MCh in conjunction with pulmonary eosinophilia and increases in DC prevalence in the trachea and bronchi.”

“At 32 h after OA exposure, airway responsiveness to MCh was increased in four of seven rats that had received sensitized MNCs (p = 0.035).”

“In contrast, the ovalbumin challenge (20 mg ml−1) inhibited the bronchoconstrictions to 5‐HT (50 μg ml−1) and MCh (100 μg ml−1).”