“Quantification of transcription levels determined that oleic acid , fungal elicitor and their combinations could induce the expressions of key genes involved in betulinic acid biosynthesis , such as HMG-CoA reductase and squalene synthase.”
“The supplementation of 1.0 g/L oleic acid led to the highest increase of betulinic acid either in dry mycelia or fermentation broth by 2-fold of the control.”
“These findings indicated that oleic acid and fungal elicitor could enhance betulinic acid metabolism by up-regulating key genes expression.”