Possible Interaction: Nitric Oxide and Phenethyl Isothiocyanate

“Treatment with PEITC induced reactive oxygen species production, nitric oxide generation , and GSH depletion and triggered DNA damage response as evidenced by flow cytometry, 8-OHdG formation, and comet assay.”

“Interestingly, BITC and PEITC promoted the levels of NO production and increased the iNOS enzyme.”

“In conclusion, the Brassica plants derivatives, PEITC and, to a lesser extent, I3C inhibit the LPS/IFN-gamma-induced NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in which the PEITC had a more potent inhibitory effect.”

“The results indicated that PEITC and I3C inhibited lipopolysaccharide (LPS)- and interferon-gamma (IFN-gamma)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance with lowering the expression of iNOS protein and mRNA.”

“ PITC dose-dependently reduced the LPS-induced secretion of nitric oxide , prostaglandin E(2), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, as well as COX-2 and iNOS protein expression.”

“Our results suggest that PEITC suppresses LPS-induced NO production via inhibition of Akt activation and the resultant decrease in expression of IFN-gamma.”

“In summary, both I3C and PEITC possessed antiinflammatory effects by inhibiting the productions of NO , TNF-α, and IL-10, although the NO-inhibitory effects may have involved in different mechanisms.”

“The results indicated that I3C and PEITC inhibited NO production, and this suppression was associated with decreased production of TNF-α and IL-10 by activated macrophages.”

“ResultsWe report that combination of PEITC + SFN or CUR + SFN has a synergistic effect in down-regulating inflammation markers like TNF, IL-1, NO , PGE2.”