Possible Interaction: Nitric Oxide and Oleic Acid

“The isolated compounds Oleic acid (3), Palmitic acid (4), desmethylisoophiopogonone B [5,7-dihydroxy-3-(4′-hydroxybenzyl)-8- methyl- chromone] (5), 5,7-dihydroxy-6-methyl-3-(4′-hydroxybenzyl) chromone (7) and 10 significantly suppressed the production of NO in LPS-induced RAW 264.7 cells.”

“Studies in our laboratory using this model and nitric oxide (NO) have shown that NO inhalation is detrimental and worsens the effects of OA.”

“In fact, oleic acid impairs nitric oxide synthase activity and endothelium-dependent vasorelaxation in vitro.”

“ Oleic acid (from 10 to 100 mumol/L) caused a concentration-dependent decrease in nitric oxide synthase activity at baseline and during ATP and ionomycin (Ca2+ ionophore) stimulation.”

“Preincubation of aortic rings with 500 microM L-arginine or 100 u/ml superoxide dismutase for 10 min partly prevented the oleic acid-induced attenuation of EDR and reduction of cGMP, indicating that oleic acid may impair the L-arginine-nitric acid pathway and/or inactivate the nitric oxide.”

“Moreover, OA enhances by transforming the growth factor-β1 (TGF-β1) release via activation of NF-κB. OA causes NO production by inducing the inducible nitric oxide synthase overexpression, thereby promoting the secretions of matrix metalloproteinases-1 (MMP-1) and MMP-3.”

“ OA inhibited LPS-induced expression of iNOS and COX-2 as well as production of NO and prostaglandin E2.”

“Whether nitric oxide impairs the regulation of gas exchange in OA lung injury remains uncertain, however.”

“Consistently, the percentage of 20:4 in serum showed a highly significant increase simultaneously with a less significant decrease in oleic acid (18:1) and linoleic acid (18:2) upon 7-day exposure to 10 ppm NO 2 .”

“We found that OOA significantly suppressed the LPS-induced production of nitric oxide (NO) and inflammatory cytokines.”