Possible Interaction: Nitric Oxide and Hydroxytyrosol

“ HT-NO not only decreased blood glucose and oxidative stress but also increased NO level and deacetylase Sirtuin 1 (SIRT1) expression in DM mice and high glucose (HG)-stimulated HUVECs.”

“ HT significantly reduced oxidative (38.5-52.4% lipid peroxidation) and nitrosative stress (48.0-51.0% nitric oxide and 43.9-75.2% peroxynitrite concentration) and brain inflammatory mediators (18.6-40.6% prostaglandin E2 and 17.0-65.0% interleukin 1β concentration).”

“ Hydroxytyrosol … NO and PGE2 but did not reduce iNOS, TNF&agr; or IL‐1&bgr; gene expression in LPS‐stimulated RAW 264.7 for 18 h. … 6 h. Resveratrol and hydroxytyrosol 10 &mgr;M induced NRf2 nuclear translocation and reduced miR‐146a expression in LPS treated RAW 264.7.”

“ HT enhanced nitric oxide (NO) production in a dose-dependent manner, and the NO synthase inhibitor L-NMMA blocked HT-mediated effects on VSMCs survival.”

“ HT inhibited production of NO and PGE₂ with an IC₅₀ of 11.4 and 19.5 µM, respectively, reflecting strong anti-inflammatory activity.”

“Our results demonstrate that HT treatment decreases both, post-hypoxic reactive oxygen species and NO levels and, consequently, HIF-1α accumulation.”

“Results showed that HT remarkably suppressed the LPS (1 μg/ml) induction of NO release.”

“The results showed that treatment of THP-1 cells with HT significantly reduced LPS-stimulated NO production and ROS formation in a concentration-dependent manner.”

“Moreover, mRNA expression of inducible nitric oxide synthase and nitric oxide production were increased after HT administration.”

“Among the compounds tested, C18 esters, namely hydroxytyrosyl stearate (HtySte) and hydroxytyrosyl oleate (HtyOle), were found to decrease NO production in a concentration-dependent manner, while the other compounds, including the parent hydroxytyrosol, were ineffective in the tested concentration range (0.5-5 μM).”