Possible Interaction: Nitric Oxide and Genistein

“… GEN down-regulated CD163 and p-STAT3 expression of THP-…, increased the levels of IL-12 and nitric oxide ( NO ) in the conditioned medium, and reduced the clonogenic and sphere-forming capacities and the expression … by co-culture of THP-1 macrophages and OCSLCs in a dose-dependent manner.”

“Pretreatment with NAC, GEN , or both significantly improved ulcer indices and increased nitric oxide level and superoxide dismutase activity.”

“ Genistein inhibited insulin-stimulated tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) and attenuated downstream Akt and endothelial nitric oxide synthase (eNOS) phosphorylation, leading to a decreased nitric oxide ( NO ) production in endothelial cells.”

“ Genistein rapidly activates endothelial nitric oxide synthase and production of nitric oxide in ECs.”

“Furthermore, genistein in postmenopausal women increases plasma nitric oxide breakdown products, reduces endothelin-1 levels and improves endothelial dependent vasodilation in post-menopausal women.”

“The tyrosine kinase antagonists tyrphostin and genistein inhibited the release of NO by parasite extracts and by GPI, alone or in combination with IFN-gamma, demonstrating the involvement of one or more tyrosine kinases in the signaling cascade.”

“ Genistein , a tyrosine kinase inhibitor, resulted in a profound suppression of the IGF-I-induced release of NO.”

“Our data indicate that the LPS-stimulated increases in COX-2 protein level and NO in supernatant are reduced by pretreatment of genistein , whereas COX-1 protein level is not affected by genistein.”

“We also found that pretreatment with three flavonoids, including quercetin, genistein , and luteolin, blocked nitric oxide synthesis in a dose-dependent fashion.”

“RESULTS With ge istein in creasing, the activities of GSH-Px, NOS and the contents of NO in creased significantly (P < 0.05), while the contents of GSH, MDA decreased both in culture supernatant and cells (P < 0.05).”