Possible Interaction: NADH and Riboflavin

“When ferricyanide was used as an artificial electron acceptor, complex I activity was normal, indicating that electrons from NADH could reduce the flavin mononucleotide cofactor.”

“Graphic abstract Smaller sized/neutral flavin analogue, riboflavin (RF) exhibits faster reactivity towards both NADH and O 2 generating more amount of O 2 ∙ − and releases higher amount of iron from different ferritins, compared to its larger sized/negatively charged derivatives such as FMN and FAD.”

“Thus, deazariboflavin is reduced catalytically by NADH in the presence of the Beneckea harveyi NAD(P)H:(flavin) oxidoreductase, the reaction proceeding to an equilibrium with an equilibrium constant near unity.”

“While both NADPH and NADH are sufficient for activity, significantly more product is observed when used in combination with flavin nucleotides.”

“Molecular docking of NADH into wild type and its mutants reveal that a small size or electronegative of residue in position29 could shorten the distance of NADH and FMN , promoting the electrons transfer and resulting in the increased activity.”

“ NADH reduces a noncovalently bound FMN , and the electrons are transported further to the quinone reduction site by a 95 Å long chain of seven iron-sulfur (Fe-S) clusters.”

“Lastly, riboflavin is shown to mediate the electrochemical oxidation of the main bacterial reducing equivalent NADH.”

“On the other hand, NADH was used as electron donor to drive azoreduction and FMN could enhance the reduction activity.”

“Using stopped-flow spectroscopy, we show that reduction of the FMN cofactor by NADH in N189A MR is multiphasic, identifying at least four different reactive configurations of the MR-NADH complex.”

“In the presence of free FMN as the electron-accepting substrate, the latter reductant showed a ping-pong Bi-Bi reaction mechanism, whereas utilization of NADH is competitively inhibited by this substrate.”