Possible Interaction: NADH and Nitric Oxide

“The cardioprotective effect of NO was accompanied by reduced NADH and NADPH oxidase activities and superoxide formation, TGF-beta1 and p27 levels, JNK activation, NF-kappa B nuclear translocation, and caspase-3 activity.”

“These data suggest that NO 2 may influence several loci of erythrocyte metabolism, including accumulation of glycolytic intermediates proximal to the reaction catalyzed by glyceraldehyde-3-phosphate dehydrogenase, oxidation of NADH , NADPH, and GSH, and alteration of NADH dependent enzymes.”

“Furthermore, an inhibitory effect of NADH on metHb formation was observed with additions of oxidants such as H2O2, NO , and NaNO2.”

“Long-term incubation of mitochondrial membranes with NO resulted in a persistent inhibition of NADH :cytochrome c reductase activity, interpreted as inhibition of NADH:ubiquinone reductase (Complex I) activity, whereas succinate:cytochrome c reductase activity, including Complex II and Complex III electron transfer, remained unaffected.”

“This was associated with an inhibition of complex I NADH : ubiquinone oxidoreductase) and complex II (succinate: ubiquinone oxidoreductase) activities of the mitochondrial respiratory chain, suggesting that NO blocks mitochondrial respiration in these cells.”

“Results In this study, we found that NO affected nicotinamide adenine dinucleotide ( NADH ) and adenosine triphosphate (ATP) levels, reduced hydrogen peroxide (H 2 O 2 ) and superoxide anion (O 2 − ) contents, and slowed O 2 − production.”

“Reduction of nitrite by NADH catalyzed by Pseudomonas aeruginosa nitrite reductase is inhibited by a high concentration of nitric oxide NO.”