Possible Interaction: NADH and Doxorubicin Hydrochloride

“Here we report that the oxidation of NADH by isolated plasma membrane vesicles was inhibited, as well, by adriamycin.”

“Both NADH ferricyanide reductase and vanadate-stimulated NADH oxidation are inhibited by adriamycin , as is a cyanide insensitive ascorbate oxidase activity, whereas NADH cytochrome c reductase is not affected.”

“ Doxorubicin (5-50 microM)-stimulated enzyme-catalyzed oxidation of NADH was also diminished, but still detectable, in the presence of chromatin.”

“In the presence of Fe(III), NADH dehydrogenase-catalyzed reduction of doxorubicin caused enhancement in the content of all modified bases over control levels.”

“ NADH dehydrogenase-catalyzed reduction of doxorubicin (25-100 microM) caused hydroxyl radical production detected as methane generated from dimethyl sulfoxide; addition of isolated human chromatin to the system produced a concentration-dependent quenching of detectable hydroxyl radical formation.”

“Both adriamycin and bathophenantroline disulfonate (BPS), previously reported as transplasma membrane electron transport inhibitors, also inhibit the CUP-stimulated NADH oxidation of pig liver plasma membranes.”

“Emodin, doxorubicin and organic extracts of C. obtusifolia inhibited NADH :cytochrome c oxidoreductase activity of bovine heart mitochondrial particles and NADH:CoQ oxidoreductase activity of porcine heart mitochondrial NADH dehydrogenase, whereas juglone was stimulatory.”

“No significant effects were observed with guanine nucleotides or with neomycin, although NADH produced a modest increase and adriamycin a small inhibition of inositol phosphate production in permeabilized islets.”

“ Doxorubicin and daunorubicin inhibited the reduction of NAD+ to NADH during reverse electron transport.”