Possible Interaction: NADH and Cupric Cation

“We demonstrated that UVA-irradiated NADH induced damage to 32P-labeled DNA fragments obtained from the p53 gene in the presence of Cu(II).”

“ESR spin-trapping experiments and mass spectrometry showed that the carbon-centered radical was formed during the reaction of NADH with Cu(II).”

“Formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine by NADH increased with its concentration in the presence of Cu(II).”

“ NADH , as well as other endogenous reductants, induced DNA damage in the presence of Cu(II).”

“Using 32P-5'-end-labeled DNA fragments obtained from human p53 tumor suppressor gene and c-Ha-ras-1 protooncogene, we showed that PBQ plus NADH , and also PHQ, induced DNA damage frequently at thymine residues, in the presence of Cu(II).”

“Both activities were inhibited by Cu2+ and Cd2+ with the NADH --> NAD activity being more resistant to inhibition.”

“ NADH and ADP (not NAD+ or ATP) enhanced LADH inactivation by Cu(II).”

“Both DCHQ and DCBQ plus NADH increased 8-oxo-7,8-dihydro-2'-deoxyguanosine in calf thymus DNA in the presence of Cu(II).”

“ NADH sharply increased the effects of Cu(II) and its complexes.”