Possible Interaction: NADH and Ascorbate

“Cpd I → Cpd II reduction by ascorbate was similarly inhibited by addition of NADH , NADP(+), thio-NADP(+), or NAD(+), though with 0.5-, 0.1-, 0.1-, and 0.01-fold reduced efficiencies, respectively, in agreement with the relative binding affinities of these dinucleotides.”

“Erythrocyte ghost membranes have been shown to oxidize NADH in the presence of the AFR.”

“Through this system, ascorbate and AFR keep levels of NADH low, thereby decreasing its inhibitory effect on adenylate cyclase activity and so increasing cAMP synthesis.”

“We studied the inhibitory effects of ascorbate on both NADH and dihydrorhodamine 123 (DHR) oxidation by peroxynitrite generated in situ from 3-morpholinosydnonimineN-ethylcarbamide (SIN-1).”

“Both 500 microM ascorbate and 50 microM glutathione decreased post-anoxic hyperoxidation of NADH and improved electrical recovery in hippocampal slices.”

“The steady-state concentration of ascorbate radicals decreased in the presence of NADH.”

“Daily treatments with ascorbate , 28 nmol/dish, maintained intracellular ascorbate and reduced NADH by half.”

“Oxidation of NADH , but not xanthine, was inhibited by KCN, ascorbate , MnCl2, cytochrome c, mannitol, Tris, epinephrine, norepinephrine, and triiodothyronine.”

“The inhibitory species is DPNH and the inhibition can be reversed by pyruvate, oxaloacetate and ascorbate , all of which bring about the oxidation of DPNH.”

“Whereas 2,4-dichlorophenol gives rise to sustained oscillations at 40 μM, it was discovered that tyramine promotes damped oscillations at a concentration of 120 μM. Oxidation of NADH was completely inhibited by 4-aminophenol and ascorbate.”