Possible Interaction: NAD and Theophylline

“The … theophylline , a potent inhibitor of poly(ADP-ribose) polymerase, to the culture … of normal cells blocked over 90% of the conversion of NAD to poly(ADP-ribose) following treatment with UV or N-methyl-N'-nitro-…-nitro-soguanidine but did not affect the rate of unscheduled DNA synthesis.”

“The inhibitory effect of theophylline on NAD depletion and on [3H]thymidine incorporation was dose and cell number dependent.”

“ Theophylline , a known inhibitor of poly(ADP-ribose)polymerase, inhibited both the NAD reduction in cells treated with DNA damaging agents and the incorporation of [3H]thymidine into DNA.”

“ Theophylline (0.2 micromol x min(-1) x kg(-1) ) abolished renal response to NAD.”

“3 The P1‐purinoceptor antagonist, 8‐phenyltheophylline (10 μm), produced a large parallel rightward shift in the NAD concentration‐response curve; in contrast it produced a small parallel leftward shift in the NADP concentration‐response curve.”

“This possibility was also supported by the observation that the rate of lowering of NAD could be greatly reduced by the addition of the poly(ADP-ribose) polymerase inhibitor, theophylline , to the culture medium.”

“It was shown in acute experiments on rats … one hour after an intraperitoneal injection of theophylline (50 mg/kg) there … a decrease in the NAD + NADP content by 19.4%, a tendency to a fall of NAD.… + NADP.H2 was expressed, and the total nicotinamide coferment level was reduced.”