Possible Interaction: NAD and Nitric Oxide

“Perturbations of Na /K -ATPase and NO are implicated as major contributors to the early metabolic and functionally reversible phase of DPN.”

“These results suggest that the expression of mitochondrial encoded subunits CO-I , CO-II and F0F1-ATPase 6) is up-regulated in response to oxygen and NO reactive species.”

“ATP and NO are connected in that the formation of NO from NOS at the PSD resulted, in the presence of NAD , in a decrease of ATP formation in the PSD.”

“ NO increases the binding of NAD , a G3PD cofactor, to G3PD and inhibits its activity as also found by others.”

“Sodium nitroprusside also stimulates the labelling of this protein by [3H-nicotinamide]NAD+, indicating that NO facilitates the formation of an adduct between this protein and NAD+.”

“Results In this study, we found that NO affected nicotinamide adenine dinucleotide (NADH) and adenosine triphosphate (ATP) levels, reduced hydrogen peroxide (H 2 O 2 ) and superoxide anion (O 2 − ) contents, and slowed O 2 − production.”

“With this result, along with the knowledge that GAPDH is stoichiometrically S-nitrosylated, the role of NO in protein modification with NAD may be viewed as the conferring of an unexpected chemical reactivity upon GAPDH, possibly due to nitrosylation of a cysteine in the enzyme active site.”

“The reduction of each mole of NAD + by NO 2 − required the utilization of approx.”