Possible Interaction: Methylene Blue and NADH

“ MB can reroute electrons in the mitochondrial electron transfer chain directly from NADH to cytochrome c, increasing the activity of complex IV and effectively promoting mitochondrial activity while mitigating oxidative stress.”

“Incubation of NADH solutions in the presence of methylene blue resulted in a time-dependent direct oxidation of NADH.”

“ MB induced DNA damage at every nucleotide in the presence of NADH and Fe(III)-ethylenediaminetetraacetic acid, which was inhibited by OH scavengers and catalase.”

“ MB significantly increased the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an oxidative DNA lesion, in the presence of NADH and metal ions.”

“UV-visible spectrometry revealed that the absorbance of oxidized form of MB at 668nm was decreased by NADH , and the addition of metal ions attenuated the spectral change.”

“ Methylene blue , while stimulating the pentose phosphate pathway, also mediated some oxidation of NADH , thus complicating the stoichiometry of the overall system.”

“We found that MB facilitates NADH oxidation, increases NAD+, and the activity of deacetylase Sirtuin 3, and reduces protein lysine acetylation in diabetic cardiac mitochondria.”

“ Methylene blue potentiates the electron transport chain by shuttling elections from NADH and FADH2 to coenzyme Q (CoQ) and cytochrome c. The purpose of this study was to determine if methylene blue could protect RGCs from noxious stimuli.”

“More MB induced an increased contribution of NADH to the PO oscillator variance, as did increased NADH influx.”