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“In guinea pigs, aminomethylpteroylglutamic acid methotrexate ) is capable of blocking the development of delayed skin hypersensitivity, the primary antibody response, and the specific febrile response to ovalbumin and diphtheria toxoid.”
The Journal of experimental medicine • 1961 | View Paper
“An antiserum to methotrexate has been produced in a sheep against a conjugate of ovalbumin and methotrexate ( MTX ) prepared using a water-soluble carbodiimide.”