Possible Interaction: Methionine and Verapamil

“In rat neonatal cardiomyocytes, verapamil blocked spontaneous activity with EC50 of 5.3·10-8 M and prolonged the APD with EC50 of 2.5·10-8M.”

“In the K562/ADR cell line, addition of verapamil to the culture medium ( 15μ M concentration) resulted in a 3‐fold decrease in Pgp expression after 72 hr exposure.”

“ Verapamil did not modify to any significant extent NE-mediated vasoconstriction, but clearly blunted the vascular action of either MET or BHT.”

“… verapamil (200 µM), … transport from M → S [Papp from (3.58 ± 0.32) to (9.66 ± 1.55) × 10–6 cm/s, p < 0.05] and lowered the … ± 3.11) to (13.35 ± 2.01) × 10–6 cm/s, p < 0.01], with a net efflux of 1.38.”

“Both DZX and VPM when added separately to the LG, led to inhibition of Met.”

“ Verapamil and diltiazem significantly reduced it to 64, 30 and 5% ( 10−7–10−5 M ) or 79, 27, 7 and 1% (10−7–10−4 M), respectively.”

“ Verapamil also depressed the N-methylation-dependent positive inotropic effect induced by methionine in the isolated Langendorff heart.”

“ Verapamil had a 17-fold more potent effect [log(IC(50), M ): basal -6.6 +/- 0.1 (n = 5); K(+) -5.4 +/- 0.3 (n = 4); all log(IC(50)) P < 0.05, basal vs. K(+)].”

“Additionally, SHRSP arteries were more sensitive to the contractile effects of mezerein, a non-phorbol ester activator of protein kinase C. Ca2+-free solution (1.0 mM EGTA) and verapamil (10–7 M) caused relaxation (approximately −60%) of contractions in response to the phorbol ester ( 10−1 M).”

“2 Amlodipine (…), verapamil (0.5‐2 × 10−4 M), diltiazem (1–8 × 10−4 M), lignocaine (0.1–… (2–6 × 10−2 M) in descending order of potency, but not nifedipine (1–5 × 10−3 M ), increased the latency and inhibited, then abolished, … potentials in a concentration‐dependent manner.”

“3 Amlodipine (0.5–… × 10−4 M), verapamil (1–2 × 10−4 M), diltiazem (1–5 × 10−4 M) and cobalt (1 × 10−3 M ), in descending order of potency, but not nifedipine (5 × 10−4 M), inhibited then abolished evoked e.j.ps in a concentration‐dependent manner.”