Possible Interaction: Methionine and Methotrexate

“3) Results: MTX significantly reduced de novo deoxythymidylate (dTMP) and methionine biosyntheses from mitochondrial-derived formate in cells, mouse liver, and bone marrow, supporting our postulation that MTX depletes mitochondrial 1C supply.”

“Pharmacokinetic studies show that MMCMs M ) with magnetic induction significantly increase the accumulation of MTX in the tumor tissue compared with the other treatments.”

“For both cell lines, methionine depletion reduces their sensitivity to a range of chemotherapy agents that interface at the level of methionine metabolism, namely temozolomide, cisplatin and methotrexate.”

“The authors conclude that methionine metabolism influences MTX neurotoxicity.”

“Remethylation of Hcy into methionine and DNA methylation are also affected by MTX treatment.”

“The median leukocyte count was higher for M (73×109/l) than for the other treatment regimens (T: 27×109/l, T+C: 37×109/l, MP: 24×109/l, MP+MTX : 30×109/l, E: 31×109/l).”

“Long-term low-dose methotrexate treatment (7.5-25 mg) inhibits production of thymidylate, purines, and methionine and leads to intracellulary accumulation of adenosin.”

“In both groups, plasma homocysteine increased and methionine decreased following administration of MTX.”

“The delay in the recovery of methionine in the intravenous 6MP group after MTX infusion is probably the result of an increased demand on methyl groups during 6MP infusion.”

“The methionine depletion may interfere with the antileukemic effect of MTX.”

“ Methotrexate reduces intracellular pools of 5-methyltetrahydrofolate and could result in reduced conversion of homocysteine to methionine by methionine synthetase.”