Possible Interaction: Methionine and Hypochlorite

“ Hypochlorous acid inactivates α-1-antitrypsin by oxidizing a critical reactive methionine residue.”

“Coincubation with either catalase, with the myeloperoxidase inhibitor azide, or with the hypochlorous acid scavenger methionine almost completely prevented activation, but not the release, of MMP-9, in supernatants of human PMNs stimulated with hiR6.”

“ HOCl can oxidize methionine to methionine sulfoxide and tyrosine to chlorotyrosine.”

“When hypochlorous acid reacted with methionine , two major products in addition to methionine sulfoxide were formed.”

“The reaction of protein with HOCl resulted in changes in protein conformation as assessed by the UV fluorescence and oxidation of the unique methionine and tyrosine, chlorination of several lysines with formation of chloramines.”

“The effects of H2O2 and HOCl were prevented by CAT and L-methionine , respectively.”

“Furthermore, we proposed a molecular model for the oxidized HypT, where methionine oxidation by HOCl results in a conformational change of the RD, inducing a counter rotation of the DBD dimers.”

“Comparative gas chromatography-coupled mass spectrometry analyses revealed that the amounts of free methionine and alanine were significantly decreased in the treated cells, demonstrating their susceptibility to hypochlorite exposure.”

“Cysteine, tryptophan, and methionine were proven to be the most vulnerable amino acids for degradation upon hypochlorous and peracetic acid treatment, while tyrosine was only prone to degradation in the presence of hypochlorous acid.”