Possible Interaction: Menthol and Calcium Supplement

“ Menthol also reduced in a concentration-dependent manner the contractile responses caused by exogenous application of Ca2+ (75-375 μM) in a Ca2+-free solution, or induced by potassium chloride (KCl; 40 mM).”

“With the use of small interfering RNA to silence the expression of TRPM8, we found that menthol could increase the concentration of intracellular calcium and decrease cell viability via the TRPM8 channel in T24 cells.”

“The principal pharmacodynamic effect of peppermint oil relevant to the gastrointestinal tract is a dose-related antispasmodic effect on the smooth musculature due to the interference of menthol with the movement of calcium across the cell membrane.”

“In summary, these findings strongly support the involvement of calcium in the penetration-enhancing effect of menthol.”

“Our data propose that menthol inhibits contractions via two mechanisms: partial blockade of Ca2+ entry via the voltage-gated, L-type calcium channels and a decrease of the calcium storage capacity of the SR.”

“The latter mechanism at least in part is mediated by the SR-resident TRPM8 channel, which by activation of menthol leads to the enhancement of passive leak of Ca2+ from the SR and reduction in the amount of the releasable calcium during activation of contractions.”

“Bath application of menthol (0.1–1 mM) had different effects on the various Ca currents present in these neurons.”

“5 Menthol (10−2 m) increased cytosolic levels of free calcium ([Ca2+]i) from 98 ± 12 to 340 ± 49 nm (P < 0.01) in fura‐2‐loaded tracheal epithelium but did not affect the intracellular adenosine 3′,5′‐cyclic monophosphate content.”

“Instead the results indicate a modulating action of menthol on Ca inactivation.”