Possible Interaction: Melatonin and Ascorbate

“Ascorbate, melatonin , and flupirtine (all at 100 microM) blunted this response in the order flupirtine > melatonin >> ascorbate.”

“The in vitro modulatory effect of melatonin on PMRS and AFR reductase activities is also reported.”

“In presence of 90 μm ascorbate , the inhibition of lipid peroxidation by melatonin was strong and had a feature of synergistic interaction.”

“While the interaction of melatonin with ascorbate indicated synergism, the joint action of melatonin and α‐tocopherol was additive.”

“Key findings: Incubation with copper‐ascorbate led to alteration in activity of Cu‐Zn superoxide dismutase and catalase which were found to be protected upon co‐incubation with melatonin (80 &mgr;M for catalase and 1 &mgr;M for others).”

“Results of isothermal titration calorimetric studies with pure Cu‐Zn superoxide dismutase … different combinations of copper chloride, ascorbic acid and melatonin suggest that when melatonin is present in … along with copper‐ascorbate , it restrains the copper‐ascorbate molecules by binding with … with scavenging the free radicals generated by them.”

“Serotonin (1-100 microM), melatonin (100 microM), and antioxidant enzymes attenuated the effects of 6-OHDA, iron plus ascorbate , or 1-methyl-4-phenylpyridinium on mitochondrial swelling and membrane potential formation.”

“Serotonin and melatonin decreased the attenuation of synaptosomal Ca(2+) uptake induced by either 6-OHDA alone or iron plus ascorbate.”

“The ascorbate induced light emission in hepatic microsomes was inhibited by melatonin treatment [control group: 10.714 ± 0.894; melatonin group: 3.162 ± 0.515, counts per minute (cpm) × 10–5].”

“Pharmacological doses of melatonin (millimolar), estrogens, pinoline and trolox (micromolar) prevented the oxidant effect of glutamate, ascorbate , and the combination of both substances.”

“ Melatonin also prevented oxidative damages by enhancing the superoxide dismutase, ascorbate peroxidase and catalase activities.”

“On the contrary, melatonin treatment in the tolerant cultivar necessitated lesser involvement of ascorbate peroxidase to deal with As-toxicity, and complemented the stress-mediated inhibition in guaiacol peroxidase activity.”

“The presence of MT significantly improved the heat tolerance of wheat seedlings by modulating their antioxidant defense system, activating the ascorbate–glutathione (AsA–GSH) cycle comprising ascorbate peroxidase (APX), and increasing glutathione reductase (GR) activities.”