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“In addition, addition of proline resulted in increased concentrations of many organic osmolytes, including glutamate, alanine, citrulline, N-acetyl-tryptophan, and mannitol , which may be beneficial for osmotic homeostasis.”
Journal of integrative plant biology • 2010 | View Paper
“Presence of NaCl in the culture medium, together with mannitol , significantly increased the level of Na+ and proline in the leaves, but it had no effect on leaf soluble sugar content.”
“A combination of 0.53 molar mannitol plus 0.1 millimolar ABA induced a proline accumulation to about 4.5 times that of control callus, equivalent to approximately 0.18 millimoles proline per gram fresh weight of callus.”