Possible Interaction: Lysine and Trypsin

“The exposure of these fibrils to trypsin generates a cleavage at the C-terminal of lysine 6 and creates the 7–99 truncated form of β2-m (ΔN6β2-m) that is a ubiquitous constituent of the natural β2-m fibrils.”

“Additionally, the presence of Arg and Lys renders the peptides susceptible to degradation by proteases, such as trypsin , limiting their therapeutic use.”

“ Trypsin is specific for the hydrolysis of amide linkages of lysine.”

“ Lysine in CPTI-II binds deeper in the specificity pocket of bovine trypsin than lysine in other known lysine-bovine-trypsin complexes, and anionic salmon trypsin lacks some of the secondary binding interactions found in the complexes formed between squash inhibitors and bovine trypsin.”

“ Trypsin PC was found to hydrolyze amide bonds formed by carboxylic groups of lysine and arginine in peptide substrates.”

“ Trypsin , Phospholipase-D and Neuraminidase at different concentrations inhibited significantly (P less than 0.001) lysine accumulation in rat intestinal cells, whereas Chymotrypsin did not show any significant effect.”

“The homologues with either lysine or arginine in the P1 position are equally good inhibitors of trypsin , plasmin and kallikrein.”

“With the L-lysine methyl esters the catalytic rate constant for hydrolysis (kcat) was found to be significantly lower for alpha-trypsin than for beta-trypsin, whereas with N2-benzoyl-L-arginine ethyl ester there was no significant difference for the two enzymes.”

“The electrophoretically pure preparation which inhibits trypsin in a specific manner is a relatively small peptide containing 5 Asp, 4 Thr, 1 Ser, 11 Glu, 6 Pro, 6 Gly, 5 Ala, 2 Val, 10 (Cys)1/2, 3 Ile, 2 Phe, 7 Lys , 3 Arg and 1 Try.”

“Also the peptides containing these methylated lysines have been proved to resist the action of trypsin and lysyl endopeptidase.”