Possible Interaction: Lysine and S-Adenosylmethionine

“Plant monofunctional aspartate kinase is unique among all aspartate kinases, showing synergistic inhibition by lysine and S‐adenosyl‐l‐methionine (SAM).”

“… binding l-lysine has been … measured with SAM bound to the cluster and l-alanine in place of l-lysine, with S-adenosyl-l-homocysteine (SAH) bound to the … to the cluster in the presence of l-alanine or of l-alanine and ethylamine in place of l-lysine.”

“A loop involved in the binding of Lys and S-adenosylmethionine provides an explanation for the synergistic inhibition by these effectors.”

“The lysine can activate one of the catecholic hydroxyl groups for a nucleophilic attack on the active methyl group of the coenzyme S-adenosyl-l-methionine (AdoMet).”

“In addition, we found that methionine analogs and high concentrations of amino acids which are known to affect SAM synthesis in other bacteria, such as threonine, lysine , and isoleucine, also caused reduced cellular levels of SAM and blocked fruiting body formation in M. xanthus.”

“Inhibition by lysine was synergistically increased by S-adenosylmethionine , which by itself had no effect on activity.”